cdk1 antibody  (NSJ Bioreagents)

Journal: iScience

Article Title: Aluminum exposure impairs nuclear envelope breakdown for mouse zygote formation

doi: 10.1016/j.isci.2026.115807

Figure Lengend Snippet: Aluminum exposure induces G2/M phase arrest in mouse zygotes (A) Representative images of γ-H2A.X in zygotes from the control group and the AlCl 3 -treated group by immunofluorescence staining. Green, γ-H2A.X; blue, DNA. Scale bars, 20 μm. (B) Relative fluorescence intensity of γ-H2A.X. Control, n = 40; AlCl 3 , n = 41; ∗∗ p < 0.005. (C) Expression level and statistical analysis of RAD51 protein in zygotes of the control group and AlCl 3 -treated group by western blot. ∗∗∗∗ p < 0.0001. (D) The mRNA expression levels of DNA damage-related genes were significantly downregulated in the treatment group compared with the control by RT-qPCR. ∗∗∗ p < 0.0005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001. (E) Representative fluorescence images of CHK1 signals in zygotes of the control group and AlCl 3 -treated group by immunofluorescence staining. Green, CHK1; blue, DNA. Scale bars, 20 μm. (F) Relative fluorescence intensity of CHK1. Control, n = 34; AlCl 3 , n = 33; ∗∗ p < 0.005. (G) Expression level and statistical analysis of CDC25C protein in zygotes of the control group and AlCl 3 -treated group by western blot. ∗∗∗ p < 0.001. (H) Expression level and statistical analysis of Cyclin B1 protein in zygotes of the control group and AlCl 3 -treated group by western blot. ∗∗ p < 0.01. (I) Expression level and statistical analysis of p -CDK1(T161) protein in zygotes of the control group and AlCl 3 -treated group by western blot. ∗∗ p < 0.005. (J) The mRNA expression of cell cycle regulatory genes by RT-qPCR. ∗∗∗ p < 0.001, ∗∗ p < 0.005, ∗∗ p < 0.005, ∗ p < 0.05. Data are presented as mean ± SEM. All experiments were performed in three independent biological replicates, with three technical replicates per biological replicate; mouse zygotes were used as research subjects. n represents the number of zygotes analyzed. Statistical analysis was performed using paired t tests. Asterisks indicate statistical significance: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Anti-phospho-CDK1 (Thr161) antibody , HuaBio , Cat# HA721987; RRID: N/A.

Techniques: Control, Immunofluorescence, Staining, Fluorescence, Expressing, Western Blot, Quantitative RT-PCR

Journal: iScience

Article Title: CGF induces ROS-mediated metabolic reprogramming and mitochondrial dysfunction to suppress colorectal cancer progression

doi: 10.1016/j.isci.2026.115273

Figure Lengend Snippet: CGF’s effect on cell cycle and apoptosis in CRC (A) Flow cytometry was used to analyze how CGF affects the cell cycle of HCT116 and HT29 cells at certain concentrations, with the percentage of cells in G1, S, and G2 phases shown in each panel. (B) Western blot analysis of the changes in cell cycle-related proteins CDK1, p-CDK1, and cyclin B1 in HCT116 and HT29 cells after CGF treatment. (C) RT-qPCR analysis of the relative expression levels of PUMA and NOXA genes in HCT116 and HT29 cells treated with different concentrations of CGF. (D) Western blot analysis of the changes in apoptosis-related proteins BCL2, PUMA, Noxa, C-caspase 9, and C-caspase 3 in HCT116 and HT29 cells after CGF treatment. (E) Flow cytometry was used to analyze apoptosis in HCT116 and HT29 cells treated with CGF. On the left is a representative plot showing apoptosis, utilizing Annexin V-FITC and PI double staining. Right: Analysis of early and late apoptosis in cells from each group using quantitative methods. (A–C and E) Data presentation is in the form of mean ± SEM. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: CDK1 Recombinant Rabbit Monoclonal Antibody , HUABIO , Cat # ET1607-51; RRID: AB_3740749.

Techniques: Flow Cytometry, Western Blot, Quantitative RT-PCR, Expressing, Double Staining